Hi Doug,
Like many others we freeze our freshly- made, buffered formaldehyde fix for light microscooy at -20C , but find it critcal to check and adjust the pH as needed after warming to the appropriate temperature. Repeated freeze thaws are not a good thing so aliquoting to the amount that works for you routinely is a good idea.
Cheers,
Mark
*************************************
Mark A. Sanders University of Minnesota
Program Director Twin Cities Campus
University Imaging Centers
www.uic.umn.edu
***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. *****I was talking with a student yesterday about their cells which did not look particularly good under the microscope. The discussion eventually came around to fixation. The student told me that he made up some 4% formaldehyde fixative and then froze back (20 deg C) aliquots for later use. This was something he had seem on an online research forum. In my fairly lengthy experience I have never heard of this being done and I am suspicious that this is NOT a good idea. Thoughts?
Doug
------------------------------
------------------------------ ------------------------------ Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
office: LSN 463 email: [hidden email]
voice: <a href="tel:520-626-2824" value="+15206262824" target="_blank">520-626-2824 fax: <a href="tel:520-626-2097" value="+15206262097" target="_blank">520-626-2097
http://swehsc.pharmacy.
arizona.edu/micro Home of: "Microscopy and Imaging Resources on the WWW"
UA Microscopy Alliance - http://microscopy.arizona.edu
| Free forum by Nabble | Edit this page |