Posted by
Knecht, David on
URL: http://confocal-microscopy-list.275.s1.nabble.com/aliquoting-4-formaldehyde-tp7585663p7585682.html
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What did the student make up the 4% aliquots from? If from pure 16% EM grade ampules, it is likely OK. If from standard 37% formaldehyde solution, I would be suspicious. We stopped using the 37% many years ago and never had problems since. We usually break open a 16% ampule and keep it in a brown bottle in the hood at room temp for weeks to months and then discard and open another. Dave
Dr. David Knecht
Professor
Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.
Storrs, CT 06269
860-486-2200
> On Sep 20, 2016, at 10:34 AM, Cromey, Douglas W - (dcromey) <
[hidden email]> wrote:
>
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> I was talking with a student yesterday about their cells which did not look particularly good under the microscope. The discussion eventually came around to fixation. The student told me that he made up some 4% formaldehyde fixative and then froze back (20 deg C) aliquots for later use. This was something he had seem on an online research forum. In my fairly lengthy experience I have never heard of this being done and I am suspicious that this is NOT a good idea. Thoughts?
>
> Doug
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