Re: Two Photon Microscope Low Signal Troubleshooting

Posted by Heping Yuan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Two-Photon-Microscope-Low-Signal-Troubleshooting-tp7585738p7585742.html

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Thank you all for your responses.

@ Michael:

Our Ti-Sapphire has 100 fs pulses and is tuned to 900 nm. We use only protected silver mirrors and the following lenses from Thorlabs:

Initial Beam expansion with:

https://www.thorlabs.com/thorproduct.cfm?partnumber=AC254-030-B
https://www.thorlabs.com/thorproduct.cfm?partnumber=AC254-060-B

Scan lens: https://www.thorlabs.com/thorproduct.cfm?partnumber=LSM05-BB
Tube lens: https://www.thorlabs.com/thorproduct.cfm?partnumber=AC508-400-B

Do you think this would this constitute too many achromats?

@ Craig:

I am measuring power after objective using a power meter from Thorlabs (https://www.thorlabs.com/thorproduct.cfm?partnumber=S121C) held up against output end of the objective.

@ Andreas:

For the PS Speck beads on a slide, when you say that the image is not too far off the expected values do you mean the expected spatial resolution values of the system?