Re: gated PMTs
Posted by
Mark Cannell-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/gated-PMTs-tp7585956p7585963.html
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Hi Simon
Not quite clear what " photostimulation happening from various light sources that more or less point directly into the condenser” means but it sounds like you have other illumination wavelengths that are in the bandpass of the detector? Can this not be avoided in some way with suitable detector filters? You are right, old research on PMTs suggest that if turned off, exposure to bright light is well tolerated -the main effect being an increase in dark current that takes many hours to decline. Exposure to bright light while powered on causes a large permanent loss in sensitivity if cathode or anode current limits are exceeded. Fast protection circuitry can help save the detector but the damage seems to accumulate over time so plan replacements… I see no reasons why these concerns would not apply to Gaasps.
Hope this helps
Mark
Mark B. Cannell Ph.D. FRSNZ FISHR
Professor of Cardiac Cell Biology
School of Physiology & Pharmacology
Faculty of Biomedical Sciences
University of Bristol
Bristol
BS8 1TD UK