Custom ring tirf alignment issue

Posted by Joe Ping-Lin Hsiao on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Custom-ring-tirf-alignment-issue-tp7586045.html

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Hi, we built a custom ring tirf system with multiple fast steering mirrors in the light path. Each mirror can tilt in both X and Y, so to achieve ring tirf we only need to control one of them.

The system works fine except for one issue. In our system, the laser beam goes into the objective from below. From time to time I found that the laser beam would be off the center axis, so I have to check that every time before experiments and to re-align the beam if it's off. Is this normal?