Re: Multiphoton microscope - Field of View

Posted by Reece, Jeff (NIH/NIDDK) [E] on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Multiphoton-microscope-Field-of-View-tp7586063p7586070.html

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Hi Tim,

 

If you are happy with precision of a few percent, you could use Powerpoint or similar software, and a decent printer, to print out your own crude sample on regular paper, and image it in transmitted or reflected light.  The spacing on my sample goes down to 500 microns, but your printer might do much better.

 

Cheers,

Jeff

 

From: Gary G. Li [mailto:[hidden email]]
Sent: Wednesday, November 30, 2016 3:00 PM
To: [hidden email]
Subject: Re: Multiphoton microscope - Field of View

 

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Look at something with a known size, e.g., a fluorescent slide with known grid spacing.  

 

On Wed, Nov 30, 2016 at 2:01 PM, Tim <[hidden email]> wrote:

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Hi all, sorry if this has been discussed before (which I'm sure it has) but I couldn't find any relevant discussions through search. I was wondering how does one go about measuring field of view on a custom designed multiphoton microscope?

Thanks,
Tim