Re: Multiphoton microscope - Field of View
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If you have a spare etched micrometer slide, you could "paint" the etched lines with the fluorescent marker. We have a very old, etched line slide that someone probably modified to be fluorescent at some time. Swipe the marker (or make a dye paint) over the etched area, let it dry, then carefully wipe the surface clean & at least some of the fluor should stay in the etched lines.
Tamara
Tamara Howard
Dept. of Cell Biology & Physiology
University of New Mexico
Albuquerque, NM
Sent: Wednesday, November 30, 2016 4:09 PM
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Subject: Re: Multiphoton microscope - Field of View
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You can also paint the printed grid with a fluorescent marker, to make it a crude fluorescent target.
***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. *****Hi Tim,
If you are happy with precision of a few percent, you could use Powerpoint or similar software, and a decent printer, to print out your own crude sample on regular paper, and image it in transmitted or reflected light. The spacing on my sample goes down to 500 microns, but your printer might do much better.
Cheers,
Jeff
From: Gary G. Li [mailto:[hidden email]]
Sent: Wednesday, November 30, 2016 3:00 PM
To: [hidden email]
Subject: Re: Multiphoton microscope - Field of View
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Look at something with a known size, e.g., a fluorescent slide with known grid spacing.
On Wed, Nov 30, 2016 at 2:01 PM, Tim <[hidden email]> wrote:
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Hi all, sorry if this has been discussed before (which I'm sure it has) but I couldn't find any relevant discussions through search. I was wondering how does one go about measuring field of view on a custom designed multiphoton microscope?
Thanks,
Tim
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