Re: Multiphoton microscope - Field of View
Posted by
Bill Bennett on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Multiphoton-microscope-Field-of-View-tp7586063p7586076.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
We use the Convollaria (lily of the valley) root cross-section slide that came with our old Zeiss LSM500. It gives a beautiful bright-field image, and fluoresces intensely right across most of our Mai Tai wavelengths. So you can use it to align BF and 2P captures and transformations, as previously mentioned. But we also find it very useful as a startup check for our 2P system to check detectors, beam alignment etc.
We got ours with the Zeiss, but I believe you can buy these slides quite cheaply e.g.
http://www.msmedia.com.au/convallaria-rhizome-training-slide-stained-for-fluorescence.phpBill Bennett