Re: Răspuns: AO and PI

Posted by mmodel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/no-subject-tp7586114p7586122.html

***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. *****

Hi Ozana,


Your bright field (it's not DIC) looks strange, it should be uniform without bright spots. I would start by checking your microscope, make sure you can obtain good and clear brightfield and fluorescent images of something that is definitely fluorescent (a drop of fluorescein, for example)


Mike Model