DIY solution to spinning disc banding artifact
Posted by
Gary Laevsky on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DIY-solution-to-spinning-disc-banding-artifact-tp7586155.html
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Hi All,
Well known artifact result of mismatched exposure and disc field
acquisitions.
Perking Elmer used to have a "solution" using the ProSynch box (or
something like that).
We're using a CSU21 that has the synch BNC cable output.
We have triggerable cameras (sCMOS (both Flash and Zyla)).
We're using Elements, so we can't exactly fine tune the exposures per acqusition (can any software?).
We're using a macro to spin up the disc to 5K. We can change the speed of
the disc, but momentum happens, so we can't do it instantaneously.
We need a way to synch the camera with the disc. Acquisitions are sub 30
ms.
I imaging we will take a signal off the disc and use this to trigger the camera.
Can someone please point me to a solution?
Thanks so much.
--
Best,
Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310