Hi Gary, you can also acquire a smaller region of interest ... Re: DIY solution to spinning disc banding artifact

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DIY-solution-to-spinning-disc-banding-artifact-tp7586155p7586156.html

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Hi Gary,

To go faster, you can also acquire a smaller region of interest ... orientation of ROI matters for sCMOS. If I recall correctly, FLASH ROI can be full width (X=2048), make Y less to go faster.

If NIS Elements does not let you acquire fast, try MetaMorph.

George

On 12/20/2016 5:00 AM, Gary Laevsky wrote:

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Hi All,

Well known artifact result of mismatched exposure and disc field
acquisitions.

Perking Elmer used to have a "solution" using the ProSynch box (or
something like that).

We're using a CSU21 that has the synch BNC cable output.

We have triggerable cameras (sCMOS (both Flash and Zyla)).

We're using Elements, so we can't exactly fine tune the exposures per acqusition (can any software?).
We're using a macro to spin up the disc to 5K.  We can change the speed of
the disc, but momentum happens, so we can't do it instantaneously.

We need a way to synch the camera with the disc.  Acquisitions are sub 30
ms.

I imaging we will take a signal off the disc and use this to trigger the camera.

Can someone please point me to a solution?

Thanks so much.

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