Re: Highly diffusible dye for filling neurons in vivo

Posted by Franco Del Principe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Highly-diffusible-dye-for-filling-neurons-in-vivo-tp7586162p7586164.html

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Hi again

As I supposed my post got rejected.

So you better google the citation and download the PDF yourself.

Cheers
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Hi Peter

As an old electrophysiologist of course Lucifer Yellow comes to mind.

Since I don't remember the 2P excitation wavelength I quote this paper
that has also other dyes listed:

"Measurement of two-photon excitation cross sections of
molecular fluorophores with data from 690 to 1050 nm"
C. Xu and W. W. Webb Vol. 13, No. 3/March 1996/J. Opt. Soc. Am. B 481

I have attached a PDF but I'm not sure it will be transmitted by the server.

Best regards

Franco

On 21-Dec-16 09:50, Peter Rupprecht wrote:

> ***** To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on
> http://www.imgur.com and include the link in your posting. *****
> Dear dye experts,
>
> I'm currently patching very small neurons (soma 5-10 um in diameter,
> dendrites much smaller) in living tissue. In addition to
> electrophysiological recordings, I'd like to get the morphology without
> fixing the tissue, so I add a dye (until recently mostly Alexa 594) to
> the pipette solution.
>
> However, since the neuronal processes are very small, it is very often
> only the soma and not the small dendrites that get filled by the dye.
> Switching from Alexa 594 to Alexa 488 improved things a bit (probably
> since 488 is smaller? https://en.wikipedia.org/wiki/Alexa_Fluor). But
> maybe somebody on the list has an idea of an even smaller, highly
> diffusible, non-toxic dye that I could use for this purpose. Plus, it
> should of course work with regular 2P excitation (800-950 nm).
>
> Any ideas?
>
> Best,
> Peter

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