Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/tiny-filters-tp7586646p7586649.html
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Dear confocal listserv, Want money? Work on cells?
Pilot Projects for a Human Cell Atlas ... request for applications
posted by Chan Zuckerberg Initiative
https://chanzuckerberg.com/initiatives/rfaREQUEST FOR APPLICATIONS
Pilot Projects for a Human Cell Atlas
OPPORTUNITY
The goal of the International Human Cell Atlas project is to create a
reference atlas of all cells in the healthy human body as a resource for
studies of health and disease. As part of this effort, the Chan
Zuckerberg Initiative seeks applications to support the Human Cell Atlas
community’s convergence around standards, protocols, and best practices
for the creation of a freely and openly available reference database of
healthy human cells and tissues.
Project Specifications
We expect the reference Cell Atlas to ultimately include a variety of
data types, including single-cell RNA sequencing, in situ hybridization
imaging, proteomics, metabolomics, epigenetic information, low- and
high-resolution images of biological samples, and more. For the initial
phases of the project, we are especially interested in groups that can
help evaluate protocols and best practices for the collection of
single-cell molecular data, ideally by directly comparing multiple
approaches to tissue preparation, data generation, or data analysis.
Example pilot project goals might include, but are not limited to:
Systematic comparisons of tissue preparation and preservation, sample
handling, and cell dissociation approaches for a variety of human
tissues and organs.
Systematic comparisons of sequencing technologies, protocols, and
resulting datasets.
Evaluation of experimental replicability, including comparing different
experimental procedures within the same lab, and similar procedures
across labs.
Comparison of image-based approaches, such as single-molecule
fluorescent in situ hybridization imaging (smFISH), with
sequencing-based approaches.
Comparison of different multiplexing strategies for image-based approaches.
Evaluating suitability of experimental strategies to different
computational pipelines.
Evaluation of biological replicability depending on donor age, sex, or
tissue type.
Evaluation of multiple tissues from one individual, compared to
different individuals.
Generation of new benchmark or validation datasets.
We are primarily interested in assembling a broad and biologically
compelling set of human tissues for the reference atlas, but in this
pilot phase, we will also consider proposals for obtaining data from
cell lines, organoids, or non-human animals.
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If you lack imagination, you can check out my post HCA-Stanford meeting
post for ideas for you to spin to your needs.
https://www.linkedin.com/pulse/comprehensive-binders-proposal-more-george-mcnamaraSincerely,
George
George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamarahttps://works.bepress.com/gmcnamara/75/http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650