http://confocal-microscopy-list.275.s1.nabble.com/CO2-gassing-using-Zeiss-spinning-disc-confocal-tp7586692p7586696.html
& Pump Level for the CO2 Module. Also make sure to calibrate the CO2 sensor
(in MTB as well!). I'm sure your local ZEISS representative/specialist can
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>
> Hi Brian
> Thanks so much. Only very gentle bubbling at the moment. I'll try doing as
> you suggest.
> Best wishes
> Julia
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]]
> On Behalf Of Armstrong, Brian
> Sent: 03 April 2017 16:39
> To:
[hidden email]
> Subject: Re: CO2 gassing using Zeiss spinning disc confocal
>
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> To join, leave or search the confocal microscopy listserv, go to:
>
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> Hi Julia, make sure that you see a significant amount of bubbles coming
> through the water in the humidification chamber. You can clean the small
> "stone" that the gas goes through. Also, you can get a new and different
> stone from an aquarium store that will let more gas flow through.
>
> Worked for us,
>
> Brian Armstrong PhD
> Associate Research Professor
> Developmental and Stem Cell Biology
> Diabetes and Metabolic Diseases
> Director, Light Microscopy Core
> Beckman Research Institute, City of Hope
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]]
> On Behalf Of Julia Edgar
> Sent: Monday, April 03, 2017 2:18 AM
> To:
[hidden email]
> Subject: CO2 gassing using Zeiss spinning disc confocal
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
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>
> Dear All
> I’m using a Zeiss spinning disc confocal microscope with small heated
> incubation stage and ‘gassing’ lid.
> The problem is that the pH of the media changes over time and becomes
> rather alkaline (turns blue pink). Motor proteins don’t behave normally
> when pH is not physiological, so this is potentially a big problem.
>
> Do you have any tips to ensure the 5% CO2 reaches the imaging dish (a 35
> mm Petri-dish) that sits between the incubation insert and the gassing lid?
> The tubing connecting the gassing lid to the water bottle (into which the
> CO2 is pumped) is rather long. Would it help to shorten it?
> Does the volume of water in the water bottle matter?
> How can I ensure the little inlet pipes in the gassing lid are not blocked?
>
> Many thanks
> Julia
> University of Glasgow.
>
>
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