Damage Threshold for Objective Lenses with Pulsed Lasers

Posted by V Hahn on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Damage-Threshold-for-Objective-Lenses-with-Pulsed-Lasers-tp7586701.html

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Hello,

I was wondering whether some of you have a coarse idea of the damage threshold of objective lenses when being used with pulsed laser sources.

I found some entries in this mailing list:
[1] https://lists.umn.edu/cgi-bin/wa?A2=CONFOCALMICROSCOPY;72b053a8.1001
refers to cw laser.
[2] http://confocal-microscopy-list.588098.n2.nabble.com/objective-lens-laser-damage-td7585244.html
refers to tirf configuration, where the laser is focused at the back focal plane.

more specifically, my application is two-photon imaging with a Leica HC PL APO 100x/1.40 Oil CS2 with a collimated beam at the backfocal plane. We use a Ti:Sa Laser (780 nm, 140fs, 80MHz, max 3.5W). I am mostly concerned about heating, epoxy detachement and burning at the front meniscus lens. In literature I found people using up to 500mW using this configuration...

Does someone have a transmission curve for these lenses?

Thank you!