http://confocal-microscopy-list.275.s1.nabble.com/Damage-Threshold-for-Objective-Lenses-with-Pulsed-Lasers-tp7586701p7586704.html
> *****
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> The transmission curves of these lenses are apparently Top Secret
> information as most vendors will not give them out. Some will give you the
> absorption value at specific wavelengths if you ask. Part of this depends
> on the back aperture of the objective and the magnification. A large back
> aperture helps you avoid burning the input of the objective, while a
> low-magnification lens typically has less glass and thus fewer
> surfaces/internal optics to absorb the light. In general, physically larger
> lenses will be able to handle more power. My own experience is that a
> typical 2-photon lens with coatings for the NIR can handle >500mW, although
> I generally try to keep it under this value to avoid pushing my luck. You
> can also get lenses specifically designed for laser machining that can
> handle watts of power and I've had limited success with such a lens in the
> past. (Mitutoyo NIR HR 50x air; note it is not well corrected for
> biological samples but works great on surface imaging)
>
> Craig
>
> On Wed, Apr 5, 2017 at 8:15 AM, V Hahn <
[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> Post images on
http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hello,
>>
>> I was wondering whether some of you have a coarse idea of the damage
>> threshold of objective lenses when being used with pulsed laser sources.
>>
>> I found some entries in this mailing list:
>> [1]
https://lists.umn.edu/cgi-bin/wa?A2=CONFOCALMICROSCOPY;72b053a8.1001>> refers to cw laser.
>> [2]
http://confocal-microscopy-list.588098.n2.nabble.com/>> objective-lens-laser-damage-td7585244.html
>> refers to tirf configuration, where the laser is focused at the back focal
>> plane.
>>
>> more specifically, my application is two-photon imaging with a Leica HC PL
>> APO 100x/1.40 Oil CS2 with a collimated beam at the backfocal plane. We use
>> a Ti:Sa Laser (780 nm, 140fs, 80MHz, max 3.5W). I am mostly concerned about
>> heating, epoxy detachement and burning at the front meniscus lens. In
>> literature I found people using up to 500mW using this configuration...
>>
>> Does someone have a transmission curve for these lenses?
>>
>> Thank you!
>>