Re: 0.3 NA 5x objective?

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/0-3-NA-5x-objective-tp7586785p7586789.html

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Pretty neat looking lens George, but if I'm reading that diagram right on
the mesolens page it's physically quite huge! I'm guessing that won't fit
on a 'regular' microscope?

Craig

On Wed, May 3, 2017 at 7:57 PM, George McNamara <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Mike,
>
> http://www.mesolens.com/product/technical-specifications
>
> Magnification: 4x
> Numerical aperture: 0.47
> Working distance: 3 mm
> Lateral field of view: 6mm
> Lateral resolution at 500nm: 640 nm
> Axial resolution at 500nm: 2.9 μm
>
> you can try asking Brad Amos for one of his Mesolenses.
>
> Recent ad in Science is unclear whether Prior will be selling this, or is
> simply proud to have supplied "leading-edge repeatability and precision" XY
> (and Z?):
>
> http://www.sciencemag.org/custom-publishing/new-products/3d-microscope
>
> The Mesolens microscope makes possible for the first time the imaging of
> relatively large biomedically important specimens such as embryos, brain
> areas, or tumors, with full 3D recording of many thousands of cells. The
> microscope’s innovative design includes a novel apochromatic lens with an
> aperture-to-magnification ratio far in excess of conventional lenses used
> by other manufacturers. This low-magnification lens, with its exceptionally
> high numerical aperture, can image an entire specimen while retaining
> subcellular detail, thus removing the need for image stitching. The focus
> motor enables the Mesolens to provide highly accurate imaging in
> the/z/-axis. The leading-edge repeatability and precision of the microscope
> stage ensures that the correct position of the specimen is obtained and
> maintained throughout the entire imaging process.  Prior Scientific
> www.prior-scientific.co.uk
>
> http://trends.directindustry.com/prior-scientific/project-14
> 131-154886.html
>
> A technical paper from the University of Strathclyde (UK) evaluating the
> Mesolens microscope may be downloaded from
>
> https://elifesciences.org/content/5/e18659/article-info.
>
> Further information about the Mesolens microscope can be found at
> www.mesolens.com.
>
> enjoy,
>
> George
>
> p.s. If Brad sends you one, please ask him to send me one too.
>
>
>
> On 5/3/2017 8:13 PM, Michael Giacomelli wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Does this exist?  Zeiss has a 0.25 5x, but I was hoping to find at
>> least 0.3 for 2 photon imaging over a wider field of view while still
>> having some axial sectioning.
>>
>> Thanks,
>> Mike
>>
>>
> --
>
>
>
>
> George McNamara, PhD
> Houston, TX 77054
> [hidden email]
> https://www.linkedin.com/in/georgemcnamara
> https://works.bepress.com/gmcnamara/75/
> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650
>