Re: 0.3 NA 5x objective?

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/0-3-NA-5x-objective-tp7586785p7586791.html

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Hi Craig,

You should ask Brad and/or his collaborator Gail McConnell
(corresponding author of their 2016 paper,
https://elifesciences.org/content/5/e18659 ).

They have it on a regular microscope in slide 29 of

http://www.innomol.eu/content/download/598/5822/file/Gail%20McConnell%20Optical%20mesoscopy%20with%20a%20new%20giant%20lens.pdf

(pdf may take several seconds to download).

enjoy,

George

p.s. hint to Nikon (whose scope is in slide 29), and other microscope
manufacturers: Mesolens or your own equivalents could be a good way to
repurpose microscope stands. How about a 6mesolens(es) light sheet
system with fusion deconvolution (and of course joint spectral unmixing)
for use with Ed Boyden's 20x^2 iExM doi:10.1038/nmeth.4261


On 5/3/2017 9:04 PM, Craig Brideau wrote:

> *****
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> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Pretty neat looking lens George, but if I'm reading that diagram right on
> the mesolens page it's physically quite huge! I'm guessing that won't fit
> on a 'regular' microscope?
>
> Craig
>
> On Wed, May 3, 2017 at 7:57 PM, George McNamara <[hidden email]>
> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi Mike,
>>
>> http://www.mesolens.com/product/technical-specifications
>>
>> Magnification: 4x
>> Numerical aperture: 0.47
>> Working distance: 3 mm
>> Lateral field of view: 6mm
>> Lateral resolution at 500nm: 640 nm
>> Axial resolution at 500nm: 2.9 μm
>>
>> you can try asking Brad Amos for one of his Mesolenses.
>>
>> Recent ad in Science is unclear whether Prior will be selling this, or is
>> simply proud to have supplied "leading-edge repeatability and precision" XY
>> (and Z?):
>>
>> http://www.sciencemag.org/custom-publishing/new-products/3d-microscope
>>
>> The Mesolens microscope makes possible for the first time the imaging of
>> relatively large biomedically important specimens such as embryos, brain
>> areas, or tumors, with full 3D recording of many thousands of cells. The
>> microscope’s innovative design includes a novel apochromatic lens with an
>> aperture-to-magnification ratio far in excess of conventional lenses used
>> by other manufacturers. This low-magnification lens, with its exceptionally
>> high numerical aperture, can image an entire specimen while retaining
>> subcellular detail, thus removing the need for image stitching. The focus
>> motor enables the Mesolens to provide highly accurate imaging in
>> the/z/-axis. The leading-edge repeatability and precision of the microscope
>> stage ensures that the correct position of the specimen is obtained and
>> maintained throughout the entire imaging process.  Prior Scientific
>> www.prior-scientific.co.uk
>>
>> http://trends.directindustry.com/prior-scientific/project-14
>> 131-154886.html
>>
>> A technical paper from the University of Strathclyde (UK) evaluating the
>> Mesolens microscope may be downloaded from
>>
>> https://elifesciences.org/content/5/e18659/article-info.
>>
>> Further information about the Mesolens microscope can be found at
>> www.mesolens.com.
>>
>> enjoy,
>>
>> George
>>
>> p.s. If Brad sends you one, please ask him to send me one too.
>>
>>
>>
>> On 5/3/2017 8:13 PM, Michael Giacomelli wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your posting.
>>> *****
>>>
>>> Does this exist?  Zeiss has a 0.25 5x, but I was hoping to find at
>>> least 0.3 for 2 photon imaging over a wider field of view while still
>>> having some axial sectioning.
>>>
>>> Thanks,
>>> Mike
>>>
>>>
>> --
>>
>>
>>
>>
>> George McNamara, PhD
>> Houston, TX 77054
>> [hidden email]
>> https://www.linkedin.com/in/georgemcnamara
>> https://works.bepress.com/gmcnamara/75/
>> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650
>>

--


George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75   (may need to use Microsoft Edge or Firefox, rather than Google Chrome)
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650