Re: 0.3 NA 5x objective?

Posted by Weber, Michael on
URL: http://confocal-microscopy-list.275.s1.nabble.com/0-3-NA-5x-objective-tp7586785p7586793.html

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Hi Michael,

The Olympus XLFLUOR 4x/0.28 air objective might work for you:

http://www.olympus-lifescience.com/en/objectives/xlfuor/

Michael

On May 4, 2017, at 1:58 AM, Csúcs Gábor <[hidden email]<mailto:[hidden email]>> wrote:

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Dear George,

I think your comment is partially misleading: they indeed have it on a regular microscope but the microscope part they use is only for sample holding/mounting and the "objective arm" is custom built. It is quite clear that the mesolens cannot be used as an easy replacement for a normal objective.

Greetings Gabor

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara
Sent: Thursday, May 4, 2017 4:16 AM
To: [hidden email]
Subject: Re: 0.3 NA 5x objective?

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Hi Craig,

You should ask Brad and/or his collaborator Gail McConnell (corresponding author of their 2016 paper,
https://elifesciences.org/content/5/e18659 ).

They have it on a regular microscope in slide 29 of

http://www.innomol.eu/content/download/598/5822/file/Gail%20McConnell%20Optical%20mesoscopy%20with%20a%20new%20giant%20lens.pdf

(pdf may take several seconds to download).

enjoy,

George

p.s. hint to Nikon (whose scope is in slide 29), and other microscope
manufacturers: Mesolens or your own equivalents could be a good way to repurpose microscope stands. How about a 6mesolens(es) light sheet system with fusion deconvolution (and of course joint spectral unmixing) for use with Ed Boyden's 20x^2 iExM doi:10.1038/nmeth.4261



_____________

Dr. Michael Weber
Advanced Microscopy Fellow
Harvard Medical School
240 Longwood Ave, LHRRB 113, Boston, MA 02115
http://nic.med.harvard.edu