Re: measure vacuole and cell volume

Posted by mmodel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Strange-Photo-conversion-tp7586797p7586811.html

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Hi Lili,

You can do that with Imaris (www.bitplane.com), Amira (https://www.fei.com/software/amira-3d-for-life-sciences/), Voloom (https://micro-dimensions.com/voloom/) and ImageJ plugins Volumest (http://lepo.it.da.ut.ee/~markkom/volumest/) and Volume Calculator (http://imagej.net/Volume_Calculator). You can also manual “integration” by summation of cell areas at each z-level multiplied by vertical step size. But remember to correct for vertical elongation or shortening

Mike


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Sent: Wednesday, May 10, 2017 9:38 AM
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Subject: measure vacuole and cell volume

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Hi all,
I have hundreds of zess confocal z stack images. Now I need to measure the volume of the cell and volume of structures inside the cell. Are there softwares or quick way to do it? Thanks!
Best regards,
LILI ZHANG