http://confocal-microscopy-list.275.s1.nabble.com/Strange-Photo-conversion-tp7586797p7586814.html
Thank you for your suggestions. Sorry I cannot log in imgur. I do live zebrafish macrophages imaging, so the shape of cells is irregular shape. Later, I also need to measure fluorescent intensity. Since I have a lot of images, so it is better for me to find a way to do automate analysis. So what can I start with if I want to learn how to automate the analysis?
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>Post images on
http://www.imgur.com and include the link in your posting.
>*****
>
>Hi Lili,
>
>There's a lot of extra information that would be useful to us to be able to
>help you with an answer. Perhaps you could upload an image of a slice to
>imgur (see the header to this email), and that will provide us with some of
>that (cell size, tissue/culture type, staining pattern, signal to noise
>ratio, etc.)
>
>Different tools will work with different efficacy depending on the
>parameters I briefly listed above, but probably using FIJI (FIJI.sc) is the
>way to go.
>
>If you don't have any experience in programming or image processing, it's
>not going to be quick this first time. If you have the intention to do a
>similar experiment again and again, it is worth investing the time now to
>learn how to automate the analysis, even if only partially, as it will save
>you hours of mind-numbing analysis in the future.
>
>Best wishes,
>Chris
>________________________________________
>From: Confocal Microscopy List <
[hidden email]> on behalf of 张莉莉 <
[hidden email]>
>Sent: Wednesday, May 10, 2017 9:37 AM
>To:
[hidden email]
>Subject: measure vacuole and cell volume
>
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>Post images on
http://www.imgur.com and include the link in your posting.
>*****
>
>Hi all,
>I have hundreds of zess confocal z stack images. Now I need to measure the volume of the cell and volume of structures inside the cell. Are there softwares or quick way to do it? Thanks!
>Best regards,
>LILI ZHANG
>