Posted by
mcammer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Trying-to-image-Cy7-5-AxioObserver-Z1-blocking-IR-tp7586976.html
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A few weeks ago we asked about finding a light source at 780ish nm for exciting Cy7.5. As always, people on this listserv provided very helpful suggestions. We were able to get a pE-4000 system with multiple LEDs but are finding that the Zeiss AxioObserver.Z1 fluorescence illumination optics appear to be cutting wavelengths above 700 nm.
We adjusted the LED powers such that 635, 660, 740, and 770 nm all have 52 mW at the end of the liquid light guide. We removed the IR filter for the autofocus system that is in the objectives nosepiece. However, the light at 740 and 770 nm is blocked from arriving at the objective lenses.
Does anyone know whether there is a filter somewhere else in the light path we could easily remove to pass these wavelengths or is this a limitation of the light train?
(graphs of the power loss posted at
http://microscopynotes.com/axioobserver/IR/ )
Thank you!!
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Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
Cell: 914-309-3270 (this is for calling, not texting) Office: Skirball 2nd Floor main office, back right
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