Posted by
Zdenek Svindrych-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Trying-to-image-Cy7-5-AxioObserver-Z1-blocking-IR-tp7586976p7586979.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
Hi Michael,
if you get the same readings before the filter cube (i.e. with the filter
turret removed), then there might be an IR blocking (heat blocking) filter
somewhere in the illumination part.
According to this drawing
http://www.zebrasc.com/Article.asp?pageclass=10201there is quite a number of elements in the light path. It could also be the
AR coating of the lenses (e.g Thorlabs VIS coating reflects up to 5% per
surface at 800nm), but the dramatic loss of intensity looks more like an
intentional IR blocking. Zeiss reps will know more...
Good luck!
zdenek
--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003)
Department of Biology,University of Virginia
409 McCormick Rd, Charlottesville, VA-22904
http://www.kcci.virginia.edu/[hidden email]
---------- Původní e-mail ----------
Od: Cammer, Michael <
[hidden email]>
Komu:
[hidden email]
Datum: 11. 7. 2017 22:01:28
Předmět: Trying to image Cy7.5 -- AxioObserver.Z1 blocking IR?
"*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
A few weeks ago we asked about finding a light source at 780ish nm for
exciting Cy7.5. As always, people on this listserv provided very helpful
suggestions. We were able to get a pE-4000 system with multiple LEDs but are
finding that the Zeiss AxioObserver.Z1 fluorescence illumination optics
appear to be cutting wavelengths above 700 nm.
We adjusted the LED powers such that 635, 660, 740, and 770 nm all have 52
mW at the end of the liquid light guide. We removed the IR filter for the
autofocus system that is in the objectives nosepiece. However, the light at
740 and 770 nm is blocked from arriving at the objective lenses.
Does anyone know whether there is a filter somewhere else in the light path
we could easily remove to pass these wavelengths or is this a limitation of
the light train?
(graphs of the power loss posted at
http://microscopynotes.com/axioobserver/IR/ )
Thank you!!
=*===========================================================*=
Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
Cell: 914-309-3270 (this is for calling, not texting) Office: Skirball 2nd
Floor main office, back right
http://ocs.med.nyu.edu/microscopy &
http://microscopynotes.com/------------------------------------------------------------
This email message, including any attachments, is for the sole use of the
intended recipient(s) and may contain information that is proprietary,
confidential, and exempt from disclosure under applicable law. Any
unauthorized review, use, disclosure, or distribution is prohibited. If you
have received this email in error please notify the sender by return email
and delete the original message. Please note, the recipient should check
this email and any attachments for the presence of viruses. The organization
accepts no liability for any damage caused by any virus transmitted by this
email.
=================================
"