http://confocal-microscopy-list.275.s1.nabble.com/Trying-to-image-Cy7-5-AxioObserver-Z1-blocking-IR-tp7586976p7586980.html
others it has to be physically removed. A Zeiss rep should know exactly
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>
> Hi Michael,
> if you get the same readings before the filter cube (i.e. with the filter
> turret removed), then there might be an IR blocking (heat blocking) filter
> somewhere in the illumination part.
>
> According to this drawing
>
>
http://www.zebrasc.com/Article.asp?pageclass=10201>
> there is quite a number of elements in the light path. It could also be the
> AR coating of the lenses (e.g Thorlabs VIS coating reflects up to 5% per
> surface at 800nm), but the dramatic loss of intensity looks more like an
> intentional IR blocking. Zeiss reps will know more...
> Good luck!
>
> zdenek
> --
> Zdenek Svindrych, Ph.D.
> W.M. Keck Center for Cellular Imaging (PLSB 003)
> Department of Biology,University of Virginia
> 409 McCormick Rd, Charlottesville, VA-22904
>
http://www.kcci.virginia.edu/>
[hidden email]
>
> ---------- Původní e-mail ----------
> Od: Cammer, Michael <
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> Komu:
[hidden email]
> Datum: 11. 7. 2017 22:01:28
> Předmět: Trying to image Cy7.5 -- AxioObserver.Z1 blocking IR?
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>
> A few weeks ago we asked about finding a light source at 780ish nm for
> exciting Cy7.5. As always, people on this listserv provided very helpful
> suggestions. We were able to get a pE-4000 system with multiple LEDs but
> are
> finding that the Zeiss AxioObserver.Z1 fluorescence illumination optics
> appear to be cutting wavelengths above 700 nm.
>
> We adjusted the LED powers such that 635, 660, 740, and 770 nm all have 52
> mW at the end of the liquid light guide. We removed the IR filter for the
> autofocus system that is in the objectives nosepiece. However, the light at
> 740 and 770 nm is blocked from arriving at the objective lenses.
>
> Does anyone know whether there is a filter somewhere else in the light path
> we could easily remove to pass these wavelengths or is this a limitation of
> the light train?
>
> (graphs of the power loss posted at
http://microscopynotes.com/> axioobserver/
> IR/ )
> Thank you!!
>
> =*===========================================================*=
> Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
> Cell: 914-309-3270 (this is for calling, not texting) Office: Skirball 2nd
> Floor main office, back right
>
http://ocs.med.nyu.edu/microscopy &
http://microscopynotes.com/>
>
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