http://confocal-microscopy-list.275.s1.nabble.com/Trying-to-image-Cy7-5-AxioObserver-Z1-blocking-IR-tp7586976p7586983.html
damage your eyes. With above 700 nm, possibly you might not even notice
it at first. I am not sure how much damage 'unvisible' red light might do.
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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> *****
>
> As others have pointed out, the strong 700 nm cutoff sounds very much like
> a hot-mirror somewhere in the path, as that is a very common cut-off for
> hot mirrors:
>
https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=6108>
https://www.edmundoptics.com/optics/optical-mirrors/specialty-mirrors/hot-mirrors/#resources>
https://www.chroma.com/products/parts/hot-mirror-26mm-50mm>
> The hot mirror should be close to the light entry port on the microscope.
> Some microscopes allow the mirror to be flipped out with a lever, while on
> others it has to be physically removed. A Zeiss rep should know exactly
> where it is in the path.
>
> Good luck,
> Ben Smith
>
>
> On Tue, Jul 11, 2017 at 7:30 PM, <
[hidden email]> wrote:
>
>> *****
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>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> Post images on
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>> *****
>>
>> Hi Michael,
>> if you get the same readings before the filter cube (i.e. with the filter
>> turret removed), then there might be an IR blocking (heat blocking) filter
>> somewhere in the illumination part.
>>
>> According to this drawing
>>
>>
http://www.zebrasc.com/Article.asp?pageclass=10201>>
>> there is quite a number of elements in the light path. It could also be the
>> AR coating of the lenses (e.g Thorlabs VIS coating reflects up to 5% per
>> surface at 800nm), but the dramatic loss of intensity looks more like an
>> intentional IR blocking. Zeiss reps will know more...
>> Good luck!
>>
>> zdenek
>> --
>> Zdenek Svindrych, Ph.D.
>> W.M. Keck Center for Cellular Imaging (PLSB 003)
>> Department of Biology,University of Virginia
>> 409 McCormick Rd, Charlottesville, VA-22904
>>
http://www.kcci.virginia.edu/>>
[hidden email]
>>
>> ---------- Původní e-mail ----------
>> Od: Cammer, Michael <
[hidden email]>
>> Komu:
[hidden email]
>> Datum: 11. 7. 2017 22:01:28
>> Předmět: Trying to image Cy7.5 -- AxioObserver.Z1 blocking IR?
>> "*****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> Post images on
http://www.imgur.com and include the link in your posting.
>> *****
>>
>> A few weeks ago we asked about finding a light source at 780ish nm for
>> exciting Cy7.5. As always, people on this listserv provided very helpful
>> suggestions. We were able to get a pE-4000 system with multiple LEDs but
>> are
>> finding that the Zeiss AxioObserver.Z1 fluorescence illumination optics
>> appear to be cutting wavelengths above 700 nm.
>>
>> We adjusted the LED powers such that 635, 660, 740, and 770 nm all have 52
>> mW at the end of the liquid light guide. We removed the IR filter for the
>> autofocus system that is in the objectives nosepiece. However, the light at
>> 740 and 770 nm is blocked from arriving at the objective lenses.
>>
>> Does anyone know whether there is a filter somewhere else in the light path
>> we could easily remove to pass these wavelengths or is this a limitation of
>> the light train?
>>
>> (graphs of the power loss posted at
http://microscopynotes.com/>> axioobserver/
>> IR/ )
>> Thank you!!
>>
>> =*===========================================================*=
>> Michael Cammer, DART Microscopy Laboratory, NYU Langone Medical Center
>> Cell: 914-309-3270 (this is for calling, not texting) Office: Skirball 2nd
>> Floor main office, back right
>>
http://ocs.med.nyu.edu/microscopy &
http://microscopynotes.com/>>
>>
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Steffen Dietzel, PD Dr. rer. nat