Advice on building multi-channel image splitter

Posted by Adam Glaser on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-on-building-multi-channel-image-splitter-tp7587097.html

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Hello everyone,

I was wondering if anyone could offer advice on building an in-house image splitter for microscopy.  

We are hoping to build one to split a rectangular ROI vertically into 2-4 colors.  The design seems straightforward, similar to the schematic of the Optosplit by Andor http://www.andor.com/scientific-cameras/multi-wavelength-imaging/optosplit-iii#prettyPhoto[pi_gal]/1/.

Are there any non-obvious factors to consider when building?  We will be using 2 identical tube lenses, the first to image an adjustable slit for creating the cropped rectangular ROI, the second to re-focus onto our sCMOS camera, and super resolution imaging grade dichroics from Semrock.

Thanks,
Adam