Re: Advice on building multi-channel image splitter

Posted by Michael Giacomelli on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-on-building-multi-channel-image-splitter-tp7587097p7587098.html

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Hi Adam,

The main issue we have flatness, since even very flat ones will introduce
geometric distortion.   Rather than use the high grade ones, we just
calibrate it out by imaging a grid target with white light and then using
imagej to compute the unwarp matrix.  Takes less than 100 ms per megapixel
per core to unwarp two channels in matlab this way.  Make sure you have
tip/tilt control somewhere for the dichroics as well, otherwise centering
the cameras is very painful.

Christopher Rowlands, who I think you met, also had a clever design at SPIE
this year for 6 (or maybe 8?) channel detection that balanced distortion
between channels to minimize the total error.  Not sure if you need
something like that, but you could run your design by him.

Mike

On Mon, Jul 31, 2017 at 4:11 PM, Adam Glaser <[hidden email]> wrote:

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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello everyone,
>
> I was wondering if anyone could offer advice on building an in-house image
> splitter for microscopy.
>
> We are hoping to build one to split a rectangular ROI vertically into 2-4
> colors.  The design seems straightforward, similar to the schematic of the
> Optosplit by Andor http://www.andor.com/scientific-cameras/multi-
> wavelength-imaging/optosplit-iii#prettyPhoto[pi_gal]/1/.
>
> Are there any non-obvious factors to consider when building?  We will be
> using 2 identical tube lenses, the first to image an adjustable slit for
> creating the cropped rectangular ROI, the second to re-focus onto our sCMOS
> camera, and super resolution imaging grade dichroics from Semrock.
>
> Thanks,
> Adam
>