Posted by
James Kerin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-on-building-multi-channel-image-splitter-tp7587097p7587104.html
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*Commercial response from Cairn Research Ltd - The home of emission image splitting*While it is of course possible to correct for distortion afterwards, in our opinion it's better to minimise it in the first place, as we aim to do in our products. It has to be a good scientific principle to "mess around" with your data as little as possible!We've been familiar with these issues for quite a while, and we were instrumental in Chroma Technology's introduction of "ultraflat" dichroics, in order to minimise distortion from that source.The other important consideration is to reduce differences in distortion between channels by making the optical pathways as similar as possible. We consider this to be a key advantage of our Optosplit, Triplesplit and Multisplit image splitters in particular, but it also applies to our multicamera products. Seeing is believing, so we're not afraid of any comparative tests here!
https://www.cairn-research.co.uk/products/fluorescence-detection/image-splitters/
Dr Martin Thomas, President, Cairn Research Ltd
On 07/31/2017, 09:11pm, Adam Glaser (
[hidden email]) wrote:
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Hello everyone,
I was wondering if anyone could offer advice on building an in-house image splitter for microscopy.
We are hoping to build one to split a rectangular ROI vertically into 2-4 colors. The design seems straightforward, similar to the schematic of the Optosplit by Andor
http://www.andor.com/scientific-cameras/multi-wavelength-imaging/optosplit-iii#prettyPhoto[pi_gal]/1/.
Are there any non-obvious factors to consider when building? We will be using 2 identical tube lenses, the first to image an adjustable slit for creating the cropped rectangular ROI, the second to re-focus onto our sCMOS camera, and super resolution imaging grade dichroics from Semrock.
Thanks,
Adam