Re: Advice on building multi-channel image splitter

Posted by Adam Glaser on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-on-building-multi-channel-image-splitter-tp7587097p7587109.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks everyone for the input.  Optics wise, is it important to ensure that the separated images are incident on the final tube lens parallel to the optical axis?  And simply shifted laterally to achieve the spatial offsets?  As in this cartoon illustration: http://www.andor.com/scientific-cameras/multi-wavelength-imaging/optosplit-iii#prettyPhoto[pi_gal]/1/

Or is generating the spatial offsets using a combination of tilt/tip of the dichroics and/or mirrors also adequate?  In this case the different beam paths might not all be perfectly parallel to the optical axis.

I am just thinking through my head the alignment/optimization of our home built image splitter.

Thanks again,
Adam