Re: Usefulness of super-resolution?

Posted by Tanneke den Blaauwen on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Usefulness-of-super-resolution-tp7587169p7587171.html

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Dear Gabor,

The discovery that the FtsZ-ring in bacteria is not a ring and that FtsZ is tread milling… (although the latter was also shown by 3D-SIM)

Buss, J., Coltharp, C., Huang, T., Pohlmeyer, C., Wang, S.-C., Hatem, C., & Xiao, J. (2013). In vivo organization of the FtsZ-ring by ZapA and ZapB revealed by quantitative super-resolution microscopy. Molecular Microbiology, 89(6), 1099–1120. http://doi.org/10.1111/mmi.12331
Yang, X., Lyu, Z., Miguel, A., McQuillen, R., Huang, K. C., & Xiao, J. (2017). GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis. Science (New York, NY), 355(6326), 744–747. http://doi.org/10.1126/science.aak9995 <http://doi.org/10.1126/science.aak9995>


Kind regards,

Tanneke

> On 19 Aug 2017, at 16:46, Csúcs Gábor <[hidden email]> wrote:
>
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> To join, leave or search the confocal microscopy listserv, go to:
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>
> Dear All,
>
> I would have a slightly provocative question to the community: Could you help me to identify significant/major biological discoveries that were clearly dependent on the availability of various super-resolution light microscopy methods? The PNAS paper from the Zhuang lab (2016) about the actin-spectrin ring is a good example for me but I am looking for further ones. Of course, we also use super-resolution techniques in our facility but my observation is that these are used rather to provide "one nice image for a publication" or "another piece in the puzzle of evidences" but they are not "game winners", they were not necessarily the major piece of evidence to prove a biological hypothesis. So I am looking for biological questions that could be answered "only"/mostly by the existing super-resolution methods.
> Thanks a lot for your help!
>
> Greetings Gabor
>

-------"Guardare, meravigliarsi e tornar a guardare."-------------


Dr. Tanneke den Blaauwen
Associate prof.
Bacterial Cell Biology
Swammerdam Institute for Life Sciences
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