Re: Usefulness of super-resolution?

Posted by 0000001ed7f52e4a-dmarc-request on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Usefulness-of-super-resolution-tp7587169p7587173.html

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Many surface receptors have been found to be clustered on the plasma membrane, while some were known to be clustered from electron microscopy studies, with PALM this was extended to live cells.
see e.g. Lillemeier et al. http://www.nature.com/ni/journal/v11/n1/abs/ni.1832.html?foxtrotcallback=true

We have shown on fixed cells that the B cell receptor, CD19 and CD22 are found in nanometer sized clusters.
https://www.researchgate.net/publication/236053888_The_Actin_and_Tetraspanin_Networks_Organize_Receptor_Nanoclusters_to_Regulate_B_Cell_Receptor-Mediated_Signaling
https://www.researchgate.net/publication/286924448_Nanoscale_organization_and_dynamics_of_the_siglec_CD22_cooperate_with_the_cytoskeleton_in_restraining_BCR_signalling

Well, I think there will need more to be done in this area and only time will tell if these then count as significant/major biological discoveries...

best wishes

Andreas




-----Original Message-----
From: Csúcs Gábor <[hidden email]>
To: CONFOCALMICROSCOPY <[hidden email]>
Sent: Sat, 19 Aug 2017 15:53
Subject: Usefulness of super-resolution?

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Dear All,

I would have a slightly provocative question to the community: Could you help me to identify significant/major biological discoveries that were clearly dependent on the availability of various super-resolution light microscopy methods? The PNAS paper from the Zhuang lab (2016) about the actin-spectrin ring is a good example for me but I am looking for further ones. Of course, we also use super-resolution techniques in our facility but my observation is that these are used rather to provide "one nice image for a publication" or "another piece in the puzzle of evidences" but they are not "game winners", they were not necessarily the major piece of evidence to prove a biological hypothesis. So I am looking for biological questions that could be answered "only"/mostly by the existing super-resolution methods.
        Thanks a lot for your help!

Greetings Gabor