Re: Usefulness of super-resolution?

Posted by Ewers, Helge on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Usefulness-of-super-resolution-tp7587169p7587178.html

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Hey Gabor,

there is the organisation of the y-complex within the nuclear pore (Szymborska, Science, 2013), a very important problem. The growth of dendritic spines in response to signalling, which was completely handwaving before (Tonnensen, Nature Neuroscience, 2014), and of course the amazing works on focal adhesion complexes by Waterman-Storer, Hess and Kanchanawong (Nature, 2010, Nature Cell Biology, 2015, Nature Cell Biology 2017). And the decade worth of Synaptic receptor organization by the Choquet lab. And distribution of integrins by the Giannone lab.
The groundbreaking quantitative analysis of organelle contact sites by Jennifer Lippincott Schwartz, Betzig et al, (Calm et al, Nature, 2017), the suborganization of clathrin coated pit components by Hess and Taraska (Sochacki et al, Nature Cell Biology 2017).
All these were inaccessible problems before. Although less prominent, I think it is pretty cool that we could show how individual septin complexes line up within filaments in cells (Kaplan et al., Nano Letters 2015).

Just to name a few striking and prominent examples. By now, you can find excellent biological important work i.e. in MBC all the time.

Best wishes to Zurich,

I ‘ll be there this weekend, if you want to chat,

Helge



--

Dr. Helge Ewers

Professor for Membrane Biochemistry
Freie Universität Berlin
Institute for Chemistry and Biochemistry
Thielallee 63
14195 Berlin
Germany

Joint Research Group X-Ray Microscopy
Helmholtz-Zentrum Berlin für Materialien und Energie
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On 19 Aug 2017, at 16:46, Csúcs Gábor <[hidden email]<mailto:[hidden email]>> wrote:

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Dear All,

I would have a slightly provocative question to the community: Could you help me to identify significant/major biological discoveries that were clearly dependent on the availability of various super-resolution light microscopy methods? The PNAS paper from the Zhuang lab (2016) about the actin-spectrin ring is a good example for me but I am looking for further ones. Of course, we also use super-resolution techniques in our facility but my observation is that these are used rather to provide "one nice image for a publication" or "another piece in the puzzle of evidences" but they are not "game winners", they were not necessarily the major piece of evidence to prove a biological hypothesis. So I am looking for biological questions that could be answered "only"/mostly by the existing super-resolution methods.
Thanks a lot for your help!

Greetings Gabor