Re: Usefulness of super-resolution?

Posted by Reto Fiolka on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Usefulness-of-super-resolution-tp7587169p7587182.html

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Dear Gabor and Helge,


I would not consider the quantitative analysis of organelle contact sites by Jennifer Lippincott Schwartz, Betzig et al, (Valm et al, Nature, 2017) as an application of superresolution microscopy. Per methods section, the resolution ranged from 294 microns (lateral) x 649 microns (axial) to 370 microns (lateral) x 947 microns (axial) for the wavelength range of 445-642nm.
Thus confocal-scale axial resolution and a bit poorer lateral resolution. This should by no means discredit the great effort in live multicolor imaging, it just does not fit to the list of superresolution applications.



Best,
Reto