Re: Usefulness of super-resolution?

Posted by Ewers, Helge on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Usefulness-of-super-resolution-tp7587169p7587183.html

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Hi Reto,

good to hear from you. Hope all is good at Southwestern.

Spatial resolution was not “super” strictly indeed, but the combined temporal and spatial resolution in the LLS experiments was only achievable by newly developed microscopy techniques and this is I believe an excellent showcase of what cell biology is going to be due to these newly developed approaches. So I think it is a fair answer in the spirit of Gabors question as to why we go through developing all these complicated new machines and what does the hype deliver.

More to see next week at the SMLMS2017. This time in London organised by the great Dylan Owen and Ricardo Henriques BTW. Hope to see many familiar faces.

Cheers,

Helge




--
 
 
Dr. Helge Ewers
Senior Lecturer
King's College London
Randall Division of Cell and Molecular Biophysics
 2nd Floor, Hodgkin Building
Guy's Campus
London SE1 1UL
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> On 22 Aug 2017, at 02:53, Reto Fiolka <[hidden email]> wrote:
>
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> Dear Gabor and Helge,
>
>
> I would not consider the quantitative analysis of organelle contact sites by Jennifer Lippincott Schwartz, Betzig et al, (Valm et al, Nature, 2017) as an application of superresolution microscopy. Per methods section, the resolution ranged from 294 microns (lateral) x 649 microns (axial) to 370 microns (lateral) x 947 microns (axial) for the wavelength range of 445-642nm.
> Thus confocal-scale axial resolution and a bit poorer lateral resolution. This should by no means discredit the great effort in live multicolor imaging, it just does not fit to the list of superresolution applications.
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>
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> Best,
> Reto
>