Posted by
Cole, Richard W (HEALTH) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Confocal-calibration-performance-assesment-tp7587224p7587233.html
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Javier Diez-Guerra
The light microscopy research group (
https://abrf.org/research-group/light-microscopy-research-group-lmrg) with in the ABRF (ABRF.org) has been working to develop performance testing procedures and achievable results (in conjunction with NIST). These performance tests have been performed in labs worldwide.
Here are some relevant publications: sorry for any perceived self-promotion
Stack, R., Bayles, C., Girard, A., Martin, K., Opansky, C., Schulz, K., and Cole, R. (2011) Quality Assurance Testing for Modern Optical Imaging Systems. Microscopy & Microanalysis 17(4):598-606. DOI: 10.1017/S1431927611000237
Cole, R.W., Jinadasa, T., and Brown, C.M. (2011) Resolution and Quality Control of Confocal Microscopy Optics. Nature Prot. 6 (12): 1929-1941. doi:10.1038/nprot.2011.407
Cole,R., Thibault,M., Bayles,C., Eason,B., Girard,A., Jinadasa,T., Opansky,C., Schulz,K., and Brown, C. (2013) International Test Results for Objective Lens Quality, Resolution, Spectral Accuracy and Spectral Separation for Confocal Laser Scanning Microscopes (CLSM). Microscopy & Microanalysis doi:10.1017/S1431927613013470
Jonkman, J., Brown, C.L., and Cole, R.W. (2014) Quantitative Confocal Microscopy: Beyond a Pretty Picture. Methods Cell Biol. 123:113-34 doi: 10.1016/B978-0-12-420138-5.00007-0
Brown, C., Reilly, A., and Cole, R, W. (2015) A Quantitative Measure of field Illumination. Vol 26:2 J. Biomol. Tech. doi: 10.7171/jbt.15-2602-001
BTW Mike points are "spot-on." if we could just eliminate the user ...
Cheers
Rich
Richard Cole
Research Scientist V
Director: Advanced Light Microscopy & Image Analysis Core
Wadsworth Center
Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York
120 New Scotland Avenue, Albany N.Y. 12208
518-474-7048 Phone
518-408-1730 Fax
Website
http://www.wadsworth.org/research/cores/alm twitter.com/microscopejock