http://confocal-microscopy-list.275.s1.nabble.com/Re-Confocal-calibration-performance-assesment-vendor-response-tp7587243p7587253.html
Actually, Fluor-Ref slides were invented by Dr.
P. C. Cheng (SUNY/Buffalo), originally for use in
his classroom. Because of our activities in
microscopy education, P. C. asked us to carry
them . and, to the best of my knowledge,
problem is readily solved using ND filters. To
yellow, and red excitation). However, if you are
And Geoge is correct... they are inexpensive when
compared to other reference slides. They are
Spectra, FAQs, etc. are on our website, www.microscopyeducation.com.
Microscopy/Microscopy Education ... "Education, not JustTraining"
to confocal to image analysis to SEM/TEM. Call today for details.
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>Post images on
http://www.imgur.com and include the link in your posting.
>*****
>
>Dear listserv,
>
>Fluor-Ref slides ... pick up a box or case from Chroma at a conference.
>
>As for Thermo: how about giving away THIN
>fluorescent plastic specimens .. can include
>your logo (and no, it would not fall under the
>category of pens or coffee cups that you can no
>longer give out to U.S. academic medical center staff).
>
>Back to Chroma slides ... if not going to 'Neuo'
>or 'Cell Bio', and cannot wait for M&M 8/2018 in
>Baltimore, as an alternative you can use a
>brightly colored clipboard(s). Come ot think of
>it, Thermo could give out branded clipboards as big reference slides.
>
>enjoy,
>
>George
>
>
>On 9/1/2017 1:26 PM, Kilgore, Jason A. wrote:
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>>Post images on
http://www.imgur.com and include the link in your posting.
>>*****
>>
>>
>>** Vendor response **
>>
>>Hi, Javier,
>>
>>For a uniform fluorescence standard, I
>>recommend the Fluor-Ref slides. These are sold
>>or re-sold by various places and are fairly
>>inexpensive. These are plastic slides that are
>>autofluorescent in four different wavelengths
>>and are incredibly uniform and
>>photostable. The drawbacks are that they are
>>extremely bright, so much so that you often
>>either have to throw in neutral density filters
>>or image an off-wavelength slide. Also, they
>>are thicker than a standard slide. Here's the
>>webpage from Ted
>>Pella:
>>
http://www.tedpella.com/histo_html/fluor.htm.asp
>>x or resold as a set of four from Fisher
>>Scientific:
>>
https://www.fishersci.com/shop/products/NC0158348/nc0158348#?keyword=FLUOR-REF+SET+OF+4+SLIDES>>
>>There are other published methods for
>>uniformity slides (such as a simple solution of
>>fluorescein under a coverslip, or putting dye
>>in agar) or labeling coated slides, but nothing
>>comes close to the Fluor-Ref slides for
>>photostability, storage stability, and uniformity of intensity.
>>
>>There is a good and fairly inexpensive option
>>for some of the other needs you
>>mention: FocalCheck microspheres
>>slides. There are different slides, but
>>probably Slide #1 is best for your needs,
>>catalog F36909. The top row has options where
>>you align rings of different colors to check
>>alignment, or rings and cores of the
>>microspheres. There are also TetraSpeck beads
>>of three different sizes (4, 1, and 0.5 um)
>>that are broadly fluorescent, which you can use
>>for PSF as well as alignment. The bottom row
>>has 6um beads of five different relative
>>intensities, for doing intensity calibrations.
>>Webpage from Thermo Fisher
>>Scientific:
https://www.thermofisher.com/order/catalog/product/F36909>>Manual:
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp36909.pdf>>
>>We sell fluorescent beads of smaller sizes,
>>too, if you need them (such as 20 nm) of
>>different wavelengths, for PSF measures.
>>
>>I hope this helps, but please feel free to
>>contact me offline if you wish more info (such
>>as a PowerPoint I have showing images from that last slide).
>>
>>Jason
>>
>>
>>Jason A. Kilgore
>>Technical Application Scientist
>>Molecular Probes / EVOS Tech Support
>>Thermo Fisher Scientific
>>
>>1-800-955-6288 then option 4, then option 3, then option 2.
>>Or dial direct at +1 541 335 0353
>>
[hidden email]
>>
>>This communication is intended solely for the
>>individual/entity to whom it is addressed. It
>>may contain confidential or legally privileged
>>information. Any unauthorized disclosure or
>>copying is prohibited and may be unlawful. If
>>you have received this communication in error,
>>please notify the sender immediately and delete it from your system.
>>
>>
>>
>>
>>-----Original Message-----
>>From: Confocal Microscopy List
>>[mailto:
[hidden email]] On Behalf Of Fco. Javier DÃez Guerra
>>Sent: Wednesday, August 30, 2017 5:10 AM
>>To:
[hidden email]
>>Subject: Confocal calibration & performance assesment
>>
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>>Post images on
http://www.imgur.com and include the link in your posting.
>>*****
>>
>>Dear confocalists,
>>
>>I would like to know about the preferred
>>methods used by facility managers to regularly
>>asses the performance of their confocal (and
>>wide-field) microscopes. In other words, which
>>are the most used and reliable methods to check
>>different features of confocal and wide-field microscope peformance?.
>>
>>Particularly, we'd like to check:
>>
>>- illumination uniformity across FOV, now we
>>use a slide coated with fluorescent secondary antibody.
>>
>>- XYZ Chromatic aberration (exciting 405, 488,
>>561 and 640 nm), now we use 1um beads that can be excited with all laser lines.
>>
>>- XYZ Resolution, we use smaller beads (0,17um)
>>to build and analyze PSFs (for each wavelength)
>>
>>- Laser power and stability: for power, we use
>>a power meter from Newport placed at the
>>objective exit, for stability, we capture long
>>time-series in reflexion mode with an empty preparation.
>>
>>- We do not know how to check detector (PMT,
>>GAsP, Hybrid, etc) sensitivity and SNR.
>>
>>Thanks for your input.
>>
>>
>>--
>>Fco. Javier Diez-Guerra, PhD
>>
>>Profesor Titular UAM
>>Servicio de MicroscopÃa Confocal
>>Centro de Biologia Molecular Severo Ochoa C/
>>Nicolás Cabrera, 1 Campus de Cantoblanco
>>28049 Madrid
>>SPAIN
>>
>>Tel +34 91 196 4612
>>e-mail:
[hidden email]
>
>--
>
>
>George McNamara, PhD
>Baltimore, MD 21231
>
[hidden email]
>
https://www.linkedin.com/in/georgemcnamara>
https://works.bepress.com/gmcnamara/75 (may
>need to use Microsoft Edge or Firefox, rather than Google Chrome)
>
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650>
http://confocal.jhu.edu>
>July 2017 Current Protocols article, open access:
>UNIT 4.4 Microscopy and Image Analysis
>
http://onlinelibrary.wiley.com/doi/10.1002/cphg.42/abstract>supporting materials direct link is
>
http://onlinelibrary.wiley.com/doi/10.1002/cphg.42/full#hg0404-sec-0023>figures at
>
http://onlinelibrary.wiley.com/doi/10.1002/cphg.42/figures