Posted by
Kyle Michael Douglass on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Laser-based-AF-for-Nikon-Ti-S-tp7587257p7587259.html
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My apologies: I meant to say that the objective NA should be higher than the refractive index of the mounting medium so that the laser beam may be totally internally reflected from the coverslip/medium interface.
Kyle
Dr. Kyle M. Douglass
Post-doctoral Researcher
EPFL - The Laboratory of Experimental Biophysics
http://leb.epfl.ch/http://kmdouglass.github.io________________________________________
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Sent: Tuesday, September 5, 2017 12:14 PM
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Subject: Re: Laser-based AF for Nikon Ti-S
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Hi Darren,
I don't know the specifics of the Ti-S, but if it uses an oil objective with NA higher than your mounting medium, then you could try the pgFocus open hardware system from Karl Bellvé.
http://big.umassmed.edu/wiki/index.php/PgFocusYou will need a stage or objective scanner that accepts a 0 - 10 V analog control signal and to setup the IR reference beam optics yourself.
I use this for focus lock on a 60x oil widefield setup and can maintain focus to within 5 - 10 nm standard deviation. It can sometimes be tricky to scan the stage with the focus locked, but otherwise it works well.
Cheers,
Kyle
Dr. Kyle M. Douglass
Post-doctoral Researcher
EPFL - The Laboratory of Experimental Biophysics
http://leb.epfl.ch/http://kmdouglass.github.io________________________________________
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Sent: Tuesday, September 5, 2017 11:43 AM
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Subject: Laser-based AF for Nikon Ti-S
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Hello Listserv,
We've been told by Nikon that no hardware based AutoFocus system is compatible with our Nikon Ti-S body and that we have to acquire a new microscope. Has anyone had luck, or suggest alternatives? Software based AF won't do for our throughput applications.
Many thanks for your help,
Best,
Darren Thomson
Manchester Fungal Infection Group
University of Manchester