Re: Temperature and Humidity

Posted by SUBSCRIBE CONFOCALMICROSCOPY Anonymous-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Temperature-and-Humidity-tp7587387p7587392.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks Ben for the insight.

That brings me to another question: We have a confocal and a lightsheet in
a room within the fish facility. Now the fish facility requires 26 degC for
the fish. Will it (26 deg C and humidity < 60% and both maintained quite
well) affect?

On 6 October 2017 at 13:53, Benjamin E Smith <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Right, with optics a stable temperature is more important than an absolute
> temperature, as long as the temperature is around room temp.  For humidity,
> the biggest concern is condensation damaging the optics.  Basically, the
> concern is that say the cooling had a hard time and the room is at 26oC and
> 70% humidity.  If the temperature then drops to 20oC during an AC cycle,
> the relative humidity will then be approaching 100% inside the scope, which
> will definitely damage antireflective coatings and dichroic mirrors.  That
> is why spec sheets say 60% humidity max, as this give plenty of safety
> buffer for temperature fluctuations without risking condensation.  A data
> logger is invaluable, both for your own records, and any insurance claims
> if necessary.  Also, past 60% humidity you will also notice that near-IR
> mode-locked lasers don't perform as well.
>
> -Ben Smith
>
> On Thu, Oct 5, 2017 at 10:15 PM, Balakrishnan Kannan <
> [hidden email]
> > wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear Benjamin,
> >
> > Thanks. Being in a tropical climate we have a high ambient humidity,
> > typically >70%. Some of the lab areas have the AHU that can maintain the
> > humidity around 55-60%. If I ask for lower than that it requires portable
> > dehumidifier. It adds to the power bill. So we want to be optimal so as
> to
> > maintain the optics free of mould and at the same time reducing
> > additional costs.
> >
> > Temperature can be maintained in the range of 22-23 deg C. I believe that
> > is fine as long as it does not vary much.
> >
> > Best regards,
> > Core Imaging Facility Staff
> > NTU
> >
> >
> > On 6 October 2017 at 12:07, Benjamin E Smith <
> [hidden email]>
> > wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > Post images on http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Shoot for 40% relative humidity and 21.5oC.  Humidity can normally be
> 20%
> > > to 60% while the temperature you will want to be as stable as possible
> to
> > > reduce thermal creep in the optics.  Most microscopes have a spec sheet
> > > that states the range they can optimally operate in.
> > >
> > > On Thu, Oct 5, 2017 at 8:18 PM, Balakrishnan Kannan <
> > > [hidden email]>
> > > wrote:
> > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > Post images on http://www.imgur.com and include the link in your
> > > posting.
> > > > *****
> > > >
> > > > Dear Microscopists,
> > > >
> > > > Is there a standard temperature and humidity setting for an imaging
> > core
> > > > suite where confocal and wide-field microscopes are stationed?
> > > >
> > > > Thanks for your time.
> > > >
> > > > Best regards,
> > > > Core Imaging Facility Staff
> > > > NTU
> > > >
> > >
> > >
> > >
> > > --
> > > Benjamin E. Smith, Ph. D.
> > > Imaging Specialist, Vision Science
> > > University of California, Berkeley
> > > 195 Life Sciences Addition
> > > Berkeley, CA  94720-3200
> > > Tel  (510) 642-9712
> > > Fax (510) 643-6791
> > > e-mail: [hidden email]
> > > http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>
> > >
> >
>
>
>
> --
> Benjamin E. Smith, Ph. D.
> Imaging Specialist, Vision Science
> University of California, Berkeley
> 195 Life Sciences Addition
> Berkeley, CA  94720-3200
> Tel  (510) 642-9712
> Fax (510) 643-6791
> e-mail: [hidden email]
> http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>
>