Posted by
Pascal Lorentz-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Apotome-tile-scan-tp7587527.html
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Dear list
Not exactly a confocal question but I am sure I can get some feedback to
my problem here:
We observed a strange behavior when using our Zeiss Apotome system for
tile scanning. We acquired a 3x3 tile and can clearly see that some of
the images are brighter than others (I used a chroma plastic slide
here). A single image really pops out. If I draw a line profile over the
three images in a row I can see a jump in intensity from one image to
the next.
If I acquire the same tile again with the exact settings the pattern
changes. So this time another image pops out or if we are lucky they all
have the same intensity.
Do you have any idea why this happens? If we run a tile scan in wide
field with the Apotome removed and the correct shading correction in
place the line profile is perfectly flat.
So I assume there is an issue with the Apotome but I am not sure if this
is hardware or software related. I am also not exactly sure how to
properly correct for shading. As far as I understood we should use a
shading correction acquired in wide field mode and use this during
Apotome acquisition.
I would be very glad to hear from other people if they observed similar
things with their Apotome and if there is a solution to this problem.
Thanks a lot and best regards
Pascal