Posted by
Lisa Cameron on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Best-dual-camera-CSU-W1-Spinning-Disc-solution-tp7587628p7587634.html
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My impressions/understanding -
The Andor Dragonfly is not slow.
If anything it's faster than the disk speed on the Yokogawa W1.
To change pinhole sizes on the W1, there are 2 separate disks slid in and
out of place.
Andor has one disk and shifts the disk slightly to use 2 different size
pinholes which are contained on one disk.
Andor built their own spinning disk system incorporating the Borealis
technology - flattening the field of illumination and achieving greater
throughput of illumination signal than older models of Yokogawa.
Yokogawa W1 has some improvement in light throughput, but does not have
Borealis, as this is a patented Andor technology now implemented in the
Dragonfly.
Andor has at least 5 models - depending on diameter of pinholes (25um or
40um, or both), number of cameras, and additional options such as TIRF and
3d localization optics, but also has a spinning disk only option (called
Dragonfly 200).
The Yokogawa W1 also has several models with 2 pinhole sizes, and one or 2
cameras and can be attached to any microscope company stand (within reason,
I guess).
So, Nikon W1 is Nikon's implementation of Yokogawa's W1 system on a Nikon
stand with Elements software.
Olympus also sells their spinning disk confocal system with a Yokogawa W1
with Cell Sense software.
And other distributors will package a W1 - BioVision in the US, Visitron,
etc.
Options are based on software, laser combiner, camera choices, and
stand/objective choices.
The disk spinning speed on both the Andor Dragonfly and the Yokogawa W1 are
slower than the Yokogawa CSU-X1 because the disks are larger in size to
accommodate the pinholes being farther apart.
The increased distance between pinholes on the disk reduces cross-talk
between the holes and allows imaging at greater depth farther from the
coverslip than the Yokogawa CSU-X1.
If you have really thin samples, the Yokogawa CSU-X1 may still the best
system.
Testing options with your samples is the best way to see what will work
well for you.
For increased resolution, I recommend checking out Ricardo Henriques's lab
publication on SRRF - super-resolution radial fluctuations.
https://bitbucket.org/rhenriqueslab/nanoj-srrf/wiki/HomeIt can be implemented in many systems and software including
micromanager/ImageJ/Fiji, but has also been incorporated with Andor EMCCD
cameras - separately or on Dragonfly systems.
I think available options may depend on your geographical location as to
who are your distributors.
No commercial interest. And I do not have a W1 or Dragonfly currently, but
do have a CSU-X1.
Feel free to contact me offline -
I'd also be interested in others' impressions,
Lisa Cameron
Light Microscopy Core Facility
Duke University
[hidden email]
On Tue, Nov 28, 2017 at 2:31 PM, <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
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> *****
>
> Hi Kai,
> I don't think Andor Dragonfly is based on Yokogawa CSU-W1. It looks very
> different mechanically. I haven't yet had a chance to take a Dragonfly and
> a
> W1 apart to see if there are any common parts, though...
>
> I haven't worked with the W1 much (but I liked the old CSU-X1); the
> Dragonfly seems to be slow on some operations, such as moving the disk,
> moving the camera splitting dichroic (for the two camera version) or moving
> the camera magnification changers.
> If you really want W1, Andor does not seem to offer it anymore (pity,
> because of the nice Borealis feature). But there are other companies, add
> 3i
> to your list. So it boils down to the features of laser combiner, camera
> selection and, most importantly, the software user interface.
>
> One more note, it's quite straightforward to add OPRA-style superresolution
> (something like microlens-based MSIM, conceptually identical to Image
> Scanning Microscopy) to a spinning disk confocal, and Yokogawa implemented
> it already in 2015 (see DOI:10.1364/OE.23.015003). I hope they're working
> on
> it, in which case it should be commercially available soon :-).
>
> No commercial interest.
>
> Goo luck!
>
> zdenek
> --
> Zdenek Svindrych, Ph.D.
> Research Associate - Imaging Specialist
> Department of Biochemistry and Cell Biology
> Geisel School of Medicine at Dartmouth
> email:
[hidden email]
>
> ---------- Původní e-mail ----------
> Od: Kai Schleicher <
[hidden email]>
> Komu:
[hidden email]
> Datum: 28. 11. 2017 11:46:38
> Předmět: Best dual camera CSU-W1 Spinning-Disc solution?
> "*****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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> *****
>
> Dear list,
>
> our facility is currently looking into whats the "best" high-end CSU-W1
> Spinning-Disc solution, namely
>
> 1) Andor Dragonfly
> 2) Nikon CSU-W1
> 3) Visitron CSU-W1
>
> Besides testing in the field, it would also be ideal for us to learn from
> people that own one of these systems.
>
> So if you have one of these systems we'd greatly appreciate feedback and
> how
> they perform in your lab or facility.
>
> Thanks and cheers,
> Kai
> "
>