Re: Best dual camera CSU-W1 Spinning-Disc solution?

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URL: http://confocal-microscopy-list.275.s1.nabble.com/Best-dual-camera-CSU-W1-Spinning-Disc-solution-tp7587628p7587655.html

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Dear Kai, dear all

we have a CSU-W1 spinning disk setup provided by Nikon (microscope, autofocus and software) and Andor (EMCCD camera and CSU-W1 unit with Borealis) for more then one year.
All in all we are satisfied with the performance of the system and the flexibility the Nikon NIS software offers.

However, there are a few technical problems with the CSU-W1 which Andor could not manage to solve until now although we had several visits from engineers and they tried hard.

First, somehow there seems to be some dust inside the SD unit. Although the system has now cleaned and additionally sealed by the engineers, we see after some time dust particles again on the image.

Second, there is quite some lateral shift between two images if we change the dichroics between image acquisition (one camera). This shift is larger then should be expected  - according to Andor - and it is larger if we use the 20x objective and smaller if we acquired the same image with an 100x objective.

Third, we see some kind of weak ghost images: parts of the image structures appear again in other parts of the image but as much weaker structures. We see this with all lasers and filter combinations. The ghost images move in opposite directions if we move the sample. There seem to be some internal reflections the source of this but until now there is no solution to this problem.

It would be very usefull to hear if other places have similar problems with the CSU-W1 unit or if these are problems only with the Andor/Borealis combination? Are there solutions for these problems around?

Best wishes, Anje