Re: Best dual camera CSU-W1 Spinning-Disc solution?

Posted by Roland Nitschke on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Best-dual-camera-CSU-W1-Spinning-Disc-solution-tp7587628p7587664.html

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Dear Anje,

I have just a short  idea, if the ghost images and may be the lateral
shift are due to the dichroics mounted in the wrong orientation in the
filter changer. may be this should be discussed but also checked again
by ANDOR or you, if you have good access to the dichroics. Also the
mirror directing the light inside the Nikon to/from the objective could
produce such kind of problems, but I doubt that Nikon could have such a
general problem not popping up everywhere. Best would be to change all
dichroics in the W1 and see if the problem remains.

I also totally agree with Michael that if not solved it has to be
exchanged (W1 unit) by Andor as it is  a major problem for a 400k€ + system.

Best wishes

Roland

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Albert-Ludwigs-University Freiburg
Life Imaging Center (LIC) in ZBSA
Microscopy and Image Analysis Platform (MIAP)
Dr. Roland Nitschke
Habsburgerstr.49
D-79104 Freiburg
Germany
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Am 05.12.2017 um 17:55 schrieb Anje Sporbert:

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>
> Dear Kai, dear all
>
> we have a CSU-W1 spinning disk setup provided by Nikon (microscope, autofocus and software) and Andor (EMCCD camera and CSU-W1 unit with Borealis) for more then one year.
> All in all we are satisfied with the performance of the system and the flexibility the Nikon NIS software offers.
>
> However, there are a few technical problems with the CSU-W1 which Andor could not manage to solve until now although we had several visits from engineers and they tried hard.
>
> First, somehow there seems to be some dust inside the SD unit. Although the system has now cleaned and additionally sealed by the engineers, we see after some time dust particles again on the image.
>
> Second, there is quite some lateral shift between two images if we change the dichroics between image acquisition (one camera). This shift is larger then should be expected  - according to Andor - and it is larger if we use the 20x objective and smaller if we acquired the same image with an 100x objective.
>
> Third, we see some kind of weak ghost images: parts of the image structures appear again in other parts of the image but as much weaker structures. We see this with all lasers and filter combinations. The ghost images move in opposite directions if we move the sample. There seem to be some internal reflections the source of this but until now there is no solution to this problem.
>
> It would be very usefull to hear if other places have similar problems with the CSU-W1 unit or if these are problems only with the Andor/Borealis combination? Are there solutions for these problems around?
>
> Best wishes, Anje
>