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WILDE Geraint on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Best-dual-camera-CSU-W1-Spinning-Disc-solution-tp7587628p7587666.html
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Commercial response from Andor
Dear all,
I would like to state that it in developing Dragonfly, a completely new design, one of our goals was to prevent the dust ingress we had sadly experienced on the Yokogawa CSU-W1. Consequently, the Dragonfly is fully sealed from dust ingress, by use of gaskets with “slot and blade” interlinked panels making up the entire enclosure. Moreover Dragonfly is passively cooled, thereby avoiding internal fans, which are a major source of contamination, as mentioned by Ivo.
While we no longer sell the W1, we maintain our commitment to customers under our obsolescence policy, in which we offer service, support and repair for a period of five years from the notice of discontinuation. If you are an Andor customer and have a dust problem on the W1, please let us know.
Regarding the question of “reflections”, we have found that the dichroic filters in W1 can be overfilled with laser illumination, leading to scattering and waveguiding into the dichroic. This can produce background artefacts in both the standard and Borealis illumination modes. We have a service solution to this issue and again would ask Andor customers to contact us for a solution.
Kind regards
Geraint Wilde
Product Manager for Microscopy Systems.
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Roland Nitschke
Sent: 07 December 2017 14:41
To:
[hidden email]
Subject: -|EXT|- Re: [CONFOCALMICROSCOPY] Best dual camera CSU-W1 Spinning-Disc solution?
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Dear Anje,
I have just a short idea, if the ghost images and may be the lateral shift are due to the dichroics mounted in the wrong orientation in the filter changer. may be this should be discussed but also checked again by ANDOR or you, if you have good access to the dichroics. Also the mirror directing the light inside the Nikon to/from the objective could produce such kind of problems, but I doubt that Nikon could have such a general problem not popping up everywhere. Best would be to change all dichroics in the W1 and see if the problem remains.
I also totally agree with Michael that if not solved it has to be exchanged (W1 unit) by Andor as it is a major problem for a 400k€ + system.
Best wishes
Roland
___________________________
Albert-Ludwigs-University Freiburg
Life Imaging Center (LIC) in ZBSA
Microscopy and Image Analysis Platform (MIAP) Dr. Roland Nitschke
Habsburgerstr.49
D-79104 Freiburg
Germany
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https://www.miap.eu/Am 05.12.2017 um 17:55 schrieb Anje Sporbert:
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>
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>
> Dear Kai, dear all
>
> we have a CSU-W1 spinning disk setup provided by Nikon (microscope, autofocus and software) and Andor (EMCCD camera and CSU-W1 unit with Borealis) for more then one year.
> All in all we are satisfied with the performance of the system and the flexibility the Nikon NIS software offers.
>
> However, there are a few technical problems with the CSU-W1 which Andor could not manage to solve until now although we had several visits from engineers and they tried hard.
>
> First, somehow there seems to be some dust inside the SD unit. Although the system has now cleaned and additionally sealed by the engineers, we see after some time dust particles again on the image.
>
> Second, there is quite some lateral shift between two images if we change the dichroics between image acquisition (one camera). This shift is larger then should be expected - according to Andor - and it is larger if we use the 20x objective and smaller if we acquired the same image with an 100x objective.
>
> Third, we see some kind of weak ghost images: parts of the image structures appear again in other parts of the image but as much weaker structures. We see this with all lasers and filter combinations. The ghost images move in opposite directions if we move the sample. There seem to be some internal reflections the source of this but until now there is no solution to this problem.
>
> It would be very usefull to hear if other places have similar problems with the CSU-W1 unit or if these are problems only with the Andor/Borealis combination? Are there solutions for these problems around?
>
> Best wishes, Anje
>
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