FLIM-FRET dye pair

Posted by Valeria Berno-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Laser-stability-over-time-tp7587681p7587683.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear all,

I don't have very much experience on FLIM-FRET technique and up to now I
had to "use" a sample with already a fluorochrome in it.

Now I have 2 users who would like to start an experiment from scratch
and I want to start with the best conditions.

Which are the best pair of recombinant protein (for
transfection/infection) and the best pair of dyes (for direct
conjugation) to start with for a FLIM FRET experiment?

We have a single photon confocal equipped with a white laser ( so pretty
much open to all wavelength) and the FLIM system (Picoquant).

thanks in advance for all your useful reply

have a nice day

Valeria


--
ALEMBIC
Advanced Light and Electron Microscopy BioImaging Center
San Raffaele Scientific Institute
DIBIT 1
via Olgettina 58, 20132 - Milano - Italy

Tel [phone]
Fax [phone]
e-mail: [hidden email]
home page: www.hsr.it/research/alembic



Rispetta l’ambiente: non stampare questa mail se non è necessario.
Respect the environment: if it's not necessary, don't print this mail.