Re: FLIM-FRET dye pair

Posted by Periasamy, Ammasi (ap3t)-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Laser-stability-over-time-tp7587681p7587685.html

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Hello Valeria,
Many people uses Cerulean-Venus or Teal(mTFP)-Venus for FLIM-FRET.
You can also use mTurquoise2-Venus. Teal and mTurquoise are more photostable than Cerulean.
Or,
You can attend our annual workshop to get trained on FLIM-FRET. If you are interested contact me off the list.
http://www.kcci.virginia.edu/workshop-2018 
Hope this helps.
Happy Holidays!
Ammasi

Dr. Ammasi Periasamy
Professor & Center Director,
WM Keck Center for Cellular Imaging,
Departments of Biology and Biomedical Engineering,
Univeristy of Virginia,
409 McCormick Rd., Charlottesville, VA 22903, USA.

http://www.kcci.virginia.edu/people/profile/ap3t
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E-mail: [hidden email]

FRET/FLIM Workshop-March 5-9, 2018: http://www.kcci.virginia.edu/workshop 



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Valeria Berno
Sent: Monday, December 18, 2017 4:05 AM
To: [hidden email]
Subject: FLIM-FRET dye pair

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Dear all,

I don't have very much experience on FLIM-FRET technique and up to now I had to "use" a sample with already a fluorochrome in it.

Now I have 2 users who would like to start an experiment from scratch and I want to start with the best conditions.

Which are the best pair of recombinant protein (for
transfection/infection) and the best pair of dyes (for direct
conjugation) to start with for a FLIM FRET experiment?

We have a single photon confocal equipped with a white laser ( so pretty much open to all wavelength) and the FLIM system (Picoquant).

thanks in advance for all your useful reply

have a nice day

Valeria


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ALEMBIC
Advanced Light and Electron Microscopy BioImaging Center San Raffaele Scientific Institute DIBIT 1 via Olgettina 58, 20132 - Milano - Italy

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