Re: FEP tubing vs BABB

Posted by Douglas Richardson on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FEP-tubing-vs-BABB-tp7588174p7588175.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Gary,

I don't think it matters whether it is or isn't.  FEP tubing has the same
RI as water.  If you put BABB inside (RI >1.5) you'll have too much
spherical aberration to see anything clearly.  Just re-hydrate the sample
into water and then place it in a microscope-friendly aqueous clearing
solution (RIMs, CUBIC-R, PROTOS, Optiview, SeeDB2, etc.)

-Doug

On Wed, Apr 25, 2018 at 10:00 AM, Gary Laevsky <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
>
> Does anyone know if FEP can stand up to BABB?  The obvious test is to put
> some tubing in BABB, but the corrosion could be slow enough that I can't
> see it, and I don't want to contaminate the objective (lightsheet
> application (and I do know this will be an optical nightmare)).
>
> Thanks.
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>