Posted by
Vitaly Boyko-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/confocal-detectors-and-deconvolution-tp7588223p7588224.html
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Hi Mike,
there is no big difference between HyDs and GaAsP detectors. Leica SMD HyDs are cooled to 18 C, thus having ca. 400 dark counts versus 2K or more for the GaAsP.I am not aware of linear deconvolution algorithms for confocal image data, thus it seems to be "cosmetic" and/or "aesthetic", and not suitable for accurate intensity based image analysis.Recent integration of FALCON combined with FCS/RICS to the Leica SP8 may bring new era to biology letting biologists quantify their data and image biological molecules at their physiological concentrations. However, most of biologists seems to be "scared" of basic equations...If you are located in the US, Leica provides much better Service and Application support compared to Zeiss. I do not have much experience with Olympus and Nikon on the confocal side.
If you have any specific questions, please let me know.
VitalyMCCF/MSKCC
On Thursday, May 10, 2018, 10:01:36 AM EDT, MODEL, MICHAEL <
[hidden email]> wrote:
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Dear colleagues,
we are looking for a new confocal microscope, and I have two questions so far.
1. Are Leica'a hybrid detectors (I am talking about SP8) in any way better than the standard GaAsP? I found an old discussion of this topic on this list, but technology may have improved since then.
2. Both Leica and Olympus 3000 use deconvolution to boost resolution. We haven't had enough time during the demos to test it thoroughly, but my impression is that some features "revealed" by deconvolution might be artefactual, so I don't know if it is worth paying extra.
Thank you
Mike Model
Kent State University