Re: Suitable laser for 2-photon brain imaging

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Suitable-laser-for-2-photon-brain-imaging-tp7588232p7588239.html

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Hi Hana, this depends heavily on what fluorophore you are using, whether
the sample is 'live' or not, etc. What is your situation?

Craig

On Fri, May 11, 2018 at 5:24 AM Hana Uhlirova <[hidden email]> wrote:

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> Hello list, I'd like to know your opinions about the laser type suitable
> for 2-photon in vivo brain imaging. We are considering the Chameleon Ultra
> family from Coherent (Ultra, Ultra I and Ultra II) and Chameleon Vision.
> With Vision we would get the dispersion pre-compensation but the peak power
> is only 2.5 W as is for the Ultra. Ultra I has peak power of 2.9 W and
> Ultra II 3.5 W. In my old lab we used to have the Ultra II which I think is
> the most common choice.
> My questions:
> 1. Does anyone use Ultra or Ultra I for multi-photon in vivo brain imaging
> and is the laser power sufficient?
> 2. Does someone use the dispersion pre-compensation of Vision I? If so, in
> which microscope and does it provide a significant improvement over a
> non-compensated beam?
> 3. What are the implications of the laser choice on fluorescence lifetime
> imaging?
>
> Thank you very much for your opinions and suggestions.
>
> Hana Uhlirova
> Institute of Scientific Instruments of the CAS
> Czech Republic
>