Posted by
Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/confocal-detectors-and-deconvolution-tp7588223p7588258.html
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Am 14.05.2018 um 07:19 schrieb George McNamara:
> line accumulation: 16
>
> frame accumulation: 16
>
> (both max'd out ... I would like Leica to enable the drop down lists
> to go to 100, or even 256, instead of max 16 ... I manage a core that
> charges by the hour, so more accum --> more revenue <--- better data).
George,
the frame accumulation I suggest to substitute with a time series and
making the sum of the images. This also gives a nice area to play
around, like: how many images you need until you can't see improvement
any more, or how random are results when images are compared. Or what
your stage drifting speed is...
Concerning the revenue, maybe you can convince the users that for
optimal images a break of X minutes between the recordings is needed, so
that the laser photons can refuel themselves :-)
Steffen
--
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging
Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany
http://www.bioimaging.bmc.med.uni-muenchen.de