Re: STEDYCON user experience/feedback?

Posted by Josef Gotzmann on
URL: http://confocal-microscopy-list.275.s1.nabble.com/STEDYCON-user-experience-feedback-tp7588285p7588286.html

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Dear Stan,

We, here in Vienna, have purchased a STEDYCON some weeks ago. We are very happy with the performance and the ease of use of the system. Without any special sample prep considerations we could reach 45nm (xy). Still, be aware of restriction on usage flexibility.
- superres for xy only
- you need really photostable dyes (STAR580 and STAR-RED work nicely in our hands)
- only orange/reddish/far-reddish dyes work with the 775nm STED laser (we currently test GFP and RFP-binding peptides, labeled with STAR dye)
- the confocal option leaves you with less crispy images than on a standard confocal (mostly due to the APDs), but the option is generally helpful
- the software needs some refinements, but I understood this is on the way. everything runs Chrome-based and the handling of data and data format needs getting used to.
- unfortunately multipositioning is only valid for the current FOV (software currently also does not support control of scanning stages)

Hope this helps.
Josef

Josef Gotzmann, Ph.D.
Central Facility BioOptics
Scientific Manager Section Light Microscopy
Max F. Perutz Laboratories Support Ges.m.b.H.
Dr. Bohrgasse 9/3; A-1030 Vienna
[hidden email]
Tel.: [phone]; mobile: [phone]
web: http://www.mfpl.ac.at/research/scientific-facilities/biooptics-light-microscopy.html?a=1
MFPL Biooptics facility e-mail: [hidden email]




On Tue, 22 May 2018 16:34:40 -0500, Stanislav Vitha <[hidden email]> wrote:

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>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Howdy,
>
>we are considering the Abberior Instruments STEDYCON shoebox STED system as an entry into superresolution for our core facility. We have not had the opportunity to see it in action yet and would appreciate your feedback/comments if you have used it or are using it currently.
>
> Thank you in advance!
>
>Best Regards,
>
>Stan Vitha
>Microscopy and Imaging Center
>Texas A&M University